Composite

Part:BBa_K2443030

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-18)
Revision as of 00:48, 19 October 2017 by Registry (Talk | contribs)

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RF2 optimized for expression in E. coli

Release factor 2 (RF2) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF2 has been Codon optimized for use in Escherichia coli and contains a N-Terminal histidine tag. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 636
    Illegal BglII site found at 1093
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
None