Part:BBa_K2295003
hre (hypoxia response element) ptal (minimalpromoter)
Hypoxia induced factors (HIFs) are transcription factors responding to decreased oxigen levels in the cellular environment. Hypoxia induced factor 1 alpha (HIF1A) is a protein constantly expressed in mammalian cells. Under normoxic conditions HIF1A is hydroxylated and is marked by the E3 ubiquitin ligase which leads to the degradation by the proteasome. However, in hypoxic conditions HIF1A is stabilized and can heterodimerize with HIF1B. Also HIF1A transcription is often significantly upregulated under hypoxic conditions. Under hypoxic conditions HIF1 can then bind to hypoxia response elements (HREs) (BBa_K2295003) in the nucleus and lead to the expression of the gene of interest (Ziello et al., 2007).
Promoter Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 51
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
This part can be seen as an improvement of BBa_K1456004 and BBa_K1720002
//function/immunology
//promoter
None |