Composite

Part:BBa_K2443010

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-18)
Revision as of 14:47, 18 October 2017 by Graeme.glaister (Talk | contribs)


IleRS optimized for expression in E. coli

isoleucine tRNA synthetase responsible for attaching isoleucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 147
    Illegal SapI.rc site found at 1538


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
None