Composite
Part:BBa_K2443004
Designed by: Lethbridge iGEM 2017 Group: iGEM17_Lethbridge (2017-10-05)
Revision as of 17:59, 5 October 2017 by Graeme.glaister (Talk | contribs)
CysRS optimized for expression in E. coli
Cysteine tRNA synthetase responsible for attaching cysteine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1042
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 908
[edit]
Categories
Parameters
//collections/cell-free_tx-tl
None |