DNA
trxL

Part:BBa_K2377005

Designed by: He Jindian   Group: iGEM17_Lanzhou   (2017-10-23)
Revision as of 12:20, 31 October 2017 by Freak9631 (Talk | contribs)


TrxzL, a developed element to generate trxz hpRNA with loop120

A developed element to generate trxz hpRNA with a normal loop, We found that the hairpin RNA actually possess higher stability and higher efficiency in gene silencing in living organisms including higher plants like Arabidopsis thaliana. In general, the hairpin RNA harbor similar effect with double-stranded RNA, what is different is that there is a loop inserted between the sense arm and anti-sense arm.

Lanzhou_wiki_Design_figure2.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 284
  • 1000
    COMPATIBLE WITH RFC[1000]


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