Translational_Unit

Part:BBa_K2505003

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)
Revision as of 23:57, 30 October 2017 by Kohei u (Talk | contribs)

IVS-IRES-log1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 225
    Illegal XhoI site found at 1275
    Illegal XhoI site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This gene is derived from Arabidopais thaliana and encode a enzyme necessary for synthesizing iP (isopentenyladenine) in mammalian cells. iP is a kind of cytokinins that are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. LOG1 has the phosphoribohydrolase activity and converts inactive cytokinin nucleotides to the biologically active free-base forms.

The DNA sequence of this genes is optimized for expressing in human cells considering the codon usage.

Characterization

We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.

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Categories
Parameters
None