Composite

Part:BBa_K2235011:Design

Designed by: Shivashree Dhanaraj and Shanlin Tong   Group: iGEM17_Stockholm   (2017-10-11)
Revision as of 20:43, 31 October 2017 by Shiva (Talk | contribs) (→‎Design Notes)


Sialidase enzyme coding composite N terminally attached to secretion system type 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3178
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3117
    Illegal XhoI site found at 127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 739
    Illegal AgeI site found at 2952
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1119
    Illegal SapI site found at 2934


Design Notes

Terminator codon was removed using PCR the following PCR primers in order to fuse the sialidase enzyme composite to the signalling peptide:
Fwd: CTAGTCTAGATAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
Rev: CTAGACTAGTTTATTGCTCAGCGGTGGCAGCAGCCAA
DNA template: Sialidase composite plasmid (BBa_K2235009).