Composite

Part:BBa_K2235011:Design

Designed by: Shivashree Dhanaraj and Shanlin Tong   Group: iGEM17_Stockholm   (2017-10-11)
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Sialidase enzyme coding composite N terminally attached to secretion system type 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3178
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3117
    Illegal XhoI site found at 127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 739
    Illegal AgeI site found at 2952
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1119
    Illegal SapI site found at 2934


Design Notes

Removal of terminator codon to attach the enzyme to the signalling peptide


Source

Reference literature

References