Part:BBa_K530008:Experience

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Team Kyoto 2017

S. cerevisiae expressing EGFP by this TDH3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 )

picture Figure1 : Observation of S. cerevisiae expressing EGFP by this TDH3 promoter with a fluorescence microscope.

B. xylophilus was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the esophagus of B. xylophilus was filled with EGFP. (fig 2 )

picture Figure2 : B. xylophilus full of EGFP in its esophagus.

This means that using TDH3 promoter makes it possible to express a very large amount of EGFP in S. cerevisiae, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding yeast. The above results indicate that BBa_K530008 (TDH 3 promoter) and BBa_K1875003(EGFP) work effectively for new usage that has not been attempted so far. Therefore, we could greatly extend the function of each part.

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