Part:BBa_K2365035

ZHD101--Zearalenone degrading enzyme (optimized)

the gene encoding ZHD101（a ZEN-degrading enzyme）was cloned. ZHD101, a lactonohydrolase produced by the fungal species Clonostachys rosea, converts ZEN into 1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen- 6-one, which is a markedly less toxic product.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

PCR and SDS-PAGE

Showing the size of the target gene and the protein.

High Performance Liquid Chromatography(HPLC) analysis:

Figure3:We use the High Performance Liquid Chromatography(HPLC) to analyze the abi lity of zearalenone(ZEN) degrading enzyme.And from articles we know that the absorption peak is about in 245 nm. The figure above shows :Data 1 represents the sample only with ZEN as contrast group ,the peak appears at 2.5 min represents ZEN and the peaks appear at 3.5min represent the isomers of ZEN.its peak area is about 27570 uV·min. The others(data2,3,4) represtents our test groups.compared with the contrast group,we preliminarily proved ZEN-degrading enzyme from the sequence (which come from GenBank AB076037 and then optimized by us) works from the discreasing peak area.