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Part:BBa_K2371014

Designed by: Wang Xiao   Group: iGEM17_BGIC-Union   (2017-10-24)
Revision as of 14:23, 24 October 2017 by Registry (Talk | contribs)

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C-t7-dCas9

dCas9 is a catalytically dead Cas9 protein that can combine with target sequence with the help of guide RNA(sgRNA). T7 RNA polymerase is a widely used polymerase from the T7 bacteriophage. BGIC-Union split the T7 polymerase into two separate parts (NT7: BBa_2371002 and CT7:BBa_2371003) and connect them to dCas9 protein via a linker. After connection with sgRNA and reconstitution of proteins, this paired report system can convert the signal of specific cancerous fused gene into various report signals in cell free system. (i.e. GFP, LacZ, RFP)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None