Deleted
Sample Discontinued
Experience: Works
Not Used
Get This Part
Device

Part:BBa_K2371013

Designed by: Qi Xiao   Group: iGEM17_BGIC-Union   (2017-10-24)
Revision as of 14:19, 24 October 2017 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


N-t7-dCas9

dCas9 is a catalytically dead Cas9 protein that can combine with target sequence with the help of guide RNA(sgRNA). T7 RNA polymerase is a widely used polymerase from the T7 bacteriophage. BGIC-Union split the T7 polymerase into two separate parts(NT7:BBa_2371002 and CT7:BBa_2371003) and connect them to dCas9 protein via a linker. After connection with sgRNA and reconstitution of proteins, this paired report system can convert the signal of specific cancerous fused gene into various report signals in cell free system. (i.e. GFP, LacZ, RFP)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2017
    Illegal BamHI site found at 1752
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2844
    Illegal NgoMIV site found at 3948
    Illegal NgoMIV site found at 4021
    Illegal NgoMIV site found at 4506
    Illegal NgoMIV site found at 5415
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None