DNA

Part:BBa_K2243011

Designed by: Li Yulong   Group: iGEM17_Peking   (2017-10-23)
Revision as of 18:59, 23 October 2017 by Registry (Talk | contribs)

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TP901-1 attL_J23119_TP901-1 attR

The constitutive promoter J23119 is between TP901-1 RDF specific sites and will be inverted by the TP901-1 recombinase and its RDF(or their fusion protein), thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase TP901-1-RDF fusion protein characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 96
    Illegal NheI site found at 119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None