Coding

Part:BBa_K2371001

Designed by: Qi Xiao   Group: iGEM17_BGIC-Union   (2017-10-22)
Revision as of 11:04, 22 October 2017 by Registry (Talk | contribs)

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C-t7-dCas9

dCas9 is a catalytically dead Cas9 protein that can combine with target sequence with the help of guide RNA(sgRNA). T7 RNA polymerase is a widely used polymerase from the T7 bacteriophage. BGIC-Union split the T7 polymerase into two separate parts (NT7: BBa_2371002 and CT7:BBa_2371003) and connect them to dCas9 protein via a linker. After connection with sgRNA and reconstitution of proteins, this paired report system can convert the signal of specific cancerous fused gene into various report signals in cell free system. (i.e. GFP, LacZ, RFP)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1238
    Illegal BamHI site found at 973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2065
    Illegal NgoMIV site found at 3169
    Illegal NgoMIV site found at 3242
    Illegal NgoMIV site found at 3727
    Illegal NgoMIV site found at 4636
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None