DNA

Part:BBa_K2243007

Designed by: Li Yulong   Group: iGEM17_Peking   (2017-10-23)
Revision as of 16:34, 23 October 2017 by Registry (Talk | contribs)

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Bxb1 attL_J23119_Bxb1 attR

The constitutive promoter J23119 is between Bxb1 gp35 specific sites and will be inverted by the Bxb1 gp35 recombinase and its RDF bxb1 gp47(or their fusion protein), thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase bxb1 gp35-gp47 fusion protein characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 96
    Illegal NheI site found at 119
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 41
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 61
    Illegal BsaI.rc site found at 160


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Categories
Parameters
None