Part:BBa_K2365511

cecropinA

BBa_K2365511 encodes Cecropin A ,a naturally occurring,linear,cationic,antimicrobial peptide,it can inhibit the growth of Fusarium graminearum.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 14
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 106
  Illegal AgeI site found at 144
  
• 1000
  COMPATIBLE WITH RFC[1000]

3-dimension structure

CecropinA (CA)is a naturally occurring, linear, cationic, 37-residue antimicrobial peptide. It has inhibitory effect on Aspergillus and Fusarium. The possible mechanism of killing bacteria is make the cell to form holes. This is the three-dimensional structure. It has the alpha helix and beta folding structure.

We did PCR and agarose gel electrophoresis to test and verify cecropinA. And then we did Tris tricine-SDS-PAGE to confirm if he successfully expressed. It’s has 177 bases and the kDa is about 5.The results show cecropinA was constructed and expressed successful.