Part:BBa_I732115

ARL4A0604

Figure I The recognition region of Lac repressor of which the amino acid residues we will try to modify. This figure comes from 'Roberto Kopke Salinas, etc. ChemBioChem 2005, 6, 1628 - 1637

The artificial repressor comes from the results by [http://parts.mit.edu/igem07/index.php/USTC/Repressor_Evolution_on_Plates Repressor Evolution on Plates]. The natural lactose repressor protein has been redesigned at Position 17, 18 of a.a. sequence, which is the composition of amino acid of recognition helix(Left Figure). Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.

Usage and Biology

• This artificial lac-repressor can bind to the specific promoters with high affinity while weakly bind to other promoters with relatively low affinity. The repression intensity is indicated in the Repression Matrix shown as below. How to acquire this repressor and how to obtain Repressor-Promoter pairs will been shown in [http://parts.mit.edu/igem07/index.php/USTC/Repressor_Evolution_on_Plates This Page].

• This repressor sequence is single repressor expression parts without other functional gene parts such as RBS & Terminators. The same repressor sequence with RBS can be found in BBa_I732915 as a Standard BioBrick part. The same repressor sequence with both RBS and Terminator can be found in BBa_I732935 as a Standard BioBrick part.

Figure II Repression Metrix(RM):The repression matrix reveals the binding affinity of different repressor candidates with various specific promoters by different colors. The deeper the red will be, the higher the repression will appear. While the lighter the blue is the weaker the repression will show.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]