Translational_Unit

Part:BBa_K2387065

Designed by: Bart Scholten   Group: iGEM17_Wageningen_UR   (2017-10-20)
Revision as of 10:21, 20 October 2017 by Registry (Talk | contribs)

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eYFP[1-154]-nZIP and eYFP[155-238]-cZIP + araC/pBAD promoter

The N-terminus of eYFP is fused to nLeucine Zipper; the C-terminus of eYFP is fused to is fused to cLeucine Zipper. The antiparallel leucine zippers have natural affinity for each other and therefore will improve the reassembly of eYFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None