Composite
Part:BBa_K2404022:Design
Designed by: Ryan Coates Group: iGEM17_Cardiff_Wales (2017-10-16)
TSH antagonist under control of the GST6 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 72
Illegal PstI site found at 1045
Illegal PstI site found at 1087 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1045
Illegal PstI site found at 1087 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1300
Illegal XhoI site found at 1112 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 72
Illegal PstI site found at 1045
Illegal PstI site found at 1087 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 72
Illegal PstI site found at 1045
Illegal PstI site found at 1087 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was put together using Golden Gate cloning, so has the appropriate restriction enzyme recognition sites in it. It is flanked by Type IIS restriction endonuclease recognition sites.
Source
The CDS was synthesised, the promoter isolated from Arabidopsis thaliana , and the terminator isolated from Agrobacterium tumefaciens