Coding

Part:BBa_K2271066:Design

Designed by: Jan Maika and Philipp Rink   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 10:31, 31 October 2017 by Jan-maika (Talk | contribs) (→‎References)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 860
    Illegal BamHI site found at 1577
    Illegal XhoI site found at 1613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The part was designed as a marker for the peroxisomal lumen.

Source

The Promoter, mRuby and Terminator were obtained from A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly from Lee et al. (2015). [2]
ePts1 was synthesized by IDT.

References

[1] Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways William C. DeLoache1,2,*, Zachary N. Russ1,2,* & John E. Dueber2


[2] A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly (2015)
Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber
ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v