Coding
Part:BBa_K2271066
Designed by: Jan Maika and Philipp Rink Group: iGEM17_Cologne-Duesseldorf (2017-10-27)
mRuby-ePTS1
Usage and Biology
This Part is a composite Part containing the fluorescent protein mRuby targeted to the peroxisome with an enhanced PTS1 described by DeLoache et al. (2016). The part is designed as a peroxisomal lumen marker for S. cerevisiea. Using the TDH3 promoter and the HHF1 terminator. For fluorometric and microscopic applications the optimal excitation of 559 nm and emission of 600 nm is discriped.
Experimental Design and Results
For the validation the S. cerevisiae Strain BY4742 was tranformed with this part. The cells were fixated and microscoped with an Elyra PS microscope. A typical peroxisomal localisation could be validated (Figure 1).
Figure 1 mRuby fused to the ePts1 described by DeLoache et al. (2016) [1.] The mRuby is localised in the peroxisomes of the cells
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 860
Illegal BamHI site found at 1577
Illegal XhoI site found at 1613 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
| None |