Composite

Part:BBa_K2404014:Design

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-10-16)
Revision as of 11:07, 16 October 2017 by Registry (Talk | contribs)

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Luc+ gene under control of the LexA promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1171
    Illegal BamHI site found at 1017
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These sequences were combined using Golden Gate cloning, and have appropriate restriction endonuclease recognition sites as a result. There are Type IIS restriction enzyme sites flanking the construct.


Source

All parts are isolated from gDNA. The promoter is from E. coli , the CDS from fireflies, and the terminator from Agrobacterium tumefaciens .

References