Composite

Part:BBa_K2311001

Designed by: Wenwen Lin   Group: iGEM17_SiCAU-China   (2017-10-21)
Revision as of 14:32, 30 October 2017 by Lww (Talk | contribs)


GFP producer controlled by 3OC6-HSL

This part has a positive feedback loop, so the expression of GFP can enhance quickly. In General, a positive feedback loop can be triggered by a low input such as PCR. This positive feedback loop will be opened by background expression of pLuxR in E.coli. But different input lead to different opening time.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2513
    Illegal NheI site found at 2536
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2501
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2282


[edit]
Categories
Parameters
None