Composite

Part:BBa_K2371008:Design

Designed by: Qi Xiao   Group: iGEM17_BGIC-Union   (2017-10-23)
Revision as of 07:58, 23 October 2017 by Registry (Talk | contribs)

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sgRNA generator for EML4-ALK variant B 70


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This coding sequence consists of the alternative target sequence of 70B and the scaffold sequence. The target sequence is complementary to one of the sites of the fusion gene EML4-ALK.


Source

DNAworks sequence synthesis

References