Coding

Part:BBa_K2336031

Designed by: Ziyang Xiao   Group: iGEM17_HUST-China   (2017-10-14)
Revision as of 17:47, 14 October 2017 by Registry (Talk | contribs)

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oprF-GS-FLAG-LBT8-GS-LBT8-GS-LBT8

The report part of our circuit. Oprf can be expressed as an anchor on the cell membrane to make sure the LBT can combine with terbium (Tb3 +) on the surface of the bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 72
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
    Illegal AgeI site found at 915
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 816


[edit]
Categories
Parameters
None