![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2443028
Designed by: Lethbridge iGEM 2017 Group: iGEM17_Lethbridge (2017-10-18)
EF-Ts optimized for expression in E. coli
EF-Ts is a prokaryotic elongation factors. EF-Ts acts at the guanine nucleotide exchange factor for EF-Tu, catalyzing the release of guanosine diphosphate from EF-Tu. This enables EF-Tu to bind to a new guanosine triphosphate molecule, release EF-Ts, and go on to catalyze another aminoacyl tRNA addition. EF-Ts has been Codon optimized for use in Escherichia coli and contains a C-terminal tag. Under the regulation of T7 Promoter, RBS and double terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 65
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//collections/cell-free_tx-tl
None |