Part:BBa_K2505005

(tra box)7-CMVmin-atipt4-IVS-IRES-log1-polyA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1442
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 26
  Illegal BglII site found at 72
  Illegal BglII site found at 118
  Illegal BglII site found at 164
  Illegal BglII site found at 210
  Illegal BglII site found at 256
  Illegal BglII site found at 302
  Illegal BamHI site found at 476
  Illegal XhoI site found at 2728
  Illegal XhoI site found at 2740
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1220
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1362
  

This part produces complete iP(isopentenyl adenine: kind of cytokinin) synthesis enzymes induced by chimeric transactivater. This part was introduced human cell(EA.hy926) then the cells produced iP when receiving signaling molecule, C8(AHL: quorum sensing signal) from E. coli. The genes(log1 and atipt4) is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase [EC:2.5.1.112]: cytokinin synthase) that necessary for a synthesis of iP(isopentenyl adenine: kind of cytokinin) precursor. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor. These enzymes are induced by TraR-C8 complex binding to tra-Box(TraR binding site:Part:BBa_K553021)

CMVmin (minimal CMV promoter: BBa_553022).