Composite

Part:BBa_K2324013

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-17)
Revision as of 17:42, 30 October 2017 by Rahannazeer (Talk | contribs)


pRha_FimH_1His

This part synthesises a FimH adhesin protein fused with a metallothionein at the first amino acid position. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a metal binding FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the fim operon .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 469
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 612


[edit]
Categories
Parameters
None