Composite

Part:BBa_K2259058

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-02)
Revision as of 18:51, 2 October 2017 by Registry (Talk | contribs)

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SynORI constitutive plasmid copy number device for group C-NC

This construct defines a constitutive copy number of plasmid by using an anderson promoter to transcribe RNA I at a specific rate. RNA I controls the copy number by binding to RNA II (plasmid replication iniator) and inhibiting its ability to form primer for polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2017
//collections/synori
Parameters
None