Regulatory
pT7

Part:BBa_K525998

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-13)
Revision as of 00:53, 22 September 2011 by Jaretz (Talk | contribs)

Promoter T7 and RBS

T7 promoter and RBS. The T7 promoter does not work with the RNA polymerase from Escherichia coli but with the RNA polymerase from the T7 phage. To express BioBricks under the control of a T7 promoter, E. coli carrying a T7 polymerase gene have to be used (e.g. BL21(DE3) or KRX).

Used to express e.g. BBa_K525123 - look there for typical induction profiles.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//direction/forward
//promoter
//regulation/positive
//rnap/bacteriophage/t7
Parameters
None