Composite

Part:BBa_K2507008

Designed by: Huanding Ji   Group: iGEM17_SHSBNU_China   (2017-10-07)
Revision as of 15:22, 9 October 2017 by HaoyuZhou (Talk | contribs)

J23105-thsR-PphsA342-sfGFP

Background

This part contain a promoter J23105, ThsR coding which can response to the result of BBa_2507012 which contain a ThsS coding, promoter PphsA342 and sfGFP coding. ThsR (BBa_K2507003) is the report part of the thiosulfate sensor which belong to the ThsS/R two-component system. This system is from S. typhimurium (Hensel et al, 1999; Price-Carter et al, 2001). Combining with BBa_2507012 which contain the ThsS coding, those two part can produce GFP after they sense the existence of thiosulfate.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1313



Results



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Parameters
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