Coding
ADH
Part:BBa_K2271115
Designed by: iGEM Cologne-Düsseldorf Group: iGEM17_Cologne-Duesseldorf (2017-10-27)
Revision as of 14:59, 30 October 2017 by PhilippMueller (Talk | contribs)
ADH alcohol dehydrogenase
The ADH we used for our project is from Pichia pastoris. It converts (+)-trans-nootkatol into (+)-nootkatone by oxidation, using NAD+ as a cofactor. Usually an additionally cofactor regeneration is not necessary, as BM3 degrades NADH+H+ into NAD+ and ADH regenerates it. But because of the improved (+)-nootkatone producing function of BM3, another cofactor regenerating enzyme is necessary. In recent studies it was shown that Glucose Dehydrogenases (GDH) are appropriate enzymes for this attempt.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 2047
Illegal BglII site found at 880 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2402
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |