Signalling

Part:BBa_K2323003

Designed by: Jorge Andres Quintero Serna   Group: iGEM17_Munich   (2017-10-25)
Revision as of 11:42, 30 October 2017 by AuroreD (Talk | contribs)


GFP-pdt2E

This GFP (E0040) is tagged with a protein degradation tag (pdt) called pdt2E. This causes the protein to degrade in E.coli with a first-order rate of ... per min, from the ClpX protease. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


[edit]
Categories
Parameters
None