Regulatory
Plac(rev)

Part:BBa_I742124

Designed by: Caroline Dahl   Group: iGEM07_Edinburgh   (2007-10-22)
Revision as of 17:23, 22 October 2007 by Caroline (Talk | contribs)

Reverse complement Lac promoter

This is an 'upside-down' version of I14032. It is a strong, constitual promoter. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional regulatory possibility that this gives.

The upstream sequence is non-functional and simply there to facilitate gel analysis or to physically enable the part to be recombineered (see the Edinburgh 2007 project where it is flanked by dif-sites).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/reverse
//chassis/prokaryote/ecoli
//promoter
//regulation/multiple
Parameters
negative_regulators1
positive_regulators