Reporter

Part:BBa_K2276007

Designed by: Tianying Chen   Group: iGEM17_SCU_China   (2017-10-24)
Revision as of 09:08, 30 October 2017 by Lilita (Talk | contribs)


a composite of TetR repressible promoter、special designed RBS and the coding sequence of mRFP

The part was designed based on the Part:BBa_I13521,while the RBS binding site is modified. The RBS is special designed for strainS whose last 9 nucleotides of the 16S rRNA is ACCTCCTTA.And it can be regard as a improvement for it has a high translation rate compared with Part:BBa_I13521.The promoter is TetR repressible promoter which is also used in the repressilator system. And the mRFP served as the reporter to help us measure the translation rate of the designed RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 634
    Illegal AgeI site found at 746
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None