Generator

Part:BBa_K2334004

Designed by: Hongbin Yu   Group: iGEM17_SCU-WestChina   (2017-10-20)
Revision as of 03:42, 21 October 2017 by Glamic (Talk | contribs)


J23100 + RiboJ + B0034 + YgfU, Urate Transporter Generator

YgfU is a high-capacity transporter for uric acid in Escherichia coli, which is homologous to nucleobase transporters of the ubiquitous family NCS2. In our project, it's used as the urate transporter in E. coli, whose expression is driven by J23100. RiboJ is used for the quantitive pathway construction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None