Composite
Part:BBa_K2323000
Designed by: Jorge Andres Quintero Serna Group: iGEM17_Munich (2017-10-25)
Revision as of 15:53, 26 October 2017 by Jorge Quintero (Talk | contribs)
Lwa Cas13a + Tphi terminator
Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules. The coding sequence is followed by Tphi terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1286
Illegal BglII site found at 2966 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2365
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Test
Functional Parameters
Test2
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Categories
Parameters
None |