Composite
Part:BBa_K2260000:Design
Designed by: Rachelle Varga Group: iGEM17_Calgary (2017-10-25)
Revision as of 07:21, 30 October 2017 by Atika ibrahim (Talk | contribs)
The phaCBA operon was codon optimized for use in E. coli using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from BBa_K1149051 were removed, including any new restriction sites introduced from codon optimization. Lastly, his tags were added to the operon.
===Source===The naturally existing phaCAB operon is present in R. eutropha H16. The operon sequence was taken from BBa_K1149051 and rearranged.