Composite

Part:BBa_K2260001

Designed by: Rachelle Varga   Group: iGEM17_Calgary   (2017-10-25)
Revision as of 07:04, 27 October 2017 by Atika ibrahim (Talk | contribs)


phaC1J4 w/ Histidine tags

Overview

E. coli (BL21) as chassis ___!!source!!____

Similar to <a href="https://parts.igem.org/Part:BBa_K2260000">BBa_K2260000</a> the part was inserted into pET29b vector downstream a T7 promoter and lac operon. Thus, the expression of the operon was induced using Isopropyl β-D-1-thiogalactopyranoside (IPTG). E. coli (BL21) was chosen as the chassis because of its ability in better protein expression. After transforming the bacteria with plasmid, colonies were selected and screened for presence of plasmid. A successful colony was then selected for experiments.

PHB from fermented "syn poo" supernatant ___!!source!!____

HPLC of PHB

Nile red suspension for confirmation of PHB

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None