Part:BBa_K2382012

EAAAK

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 428
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the target protein becomes automatically released from the fusion protein without proteolytic treatment. Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has been successfully employed for many cases of protein purification without the tag of a fusion protein.

In our design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the two proteins from interrupting each other while folding.

References

Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021