Composite

Part:BBa_K2447017

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-17)
Revision as of 01:26, 26 October 2017 by Mholowko (Talk | contribs)


Constitutive E2 killing protein production

Design of this cascade system is similar to our other part BBa_K2447016, with the addition of constitutive expression of E2 Colicin (an DNAse). E2 killing protein activity is balanced off by the controlled expression of E2 immunity protein (IM2) via appropriate phosphate and temperature inputs.

This construct is useful to serve as a highly specific kill-switch where under high phosphate concentration and/or when the temperature is higher than 36 degrees, there would be constant production of E2 immunity protein to sequester the killing protein E2. For the remaining scenario such as when the phosphate concentration is low and when temperature is lower than 36 degrees, the kill-switch would be 'activated' where the production of E2 immunity protein will be down-regulated causing accumulation of E2 Colicin and subsequent cell death.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 89
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None