![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Reporter
Part:BBa_K2333437
Designed by: Ethan M Jones Group: iGEM17_William_and_Mary (2017-10-27)
Copper sensor with Protein Degradation Tag A
This part constitutively expresses the CueR repressor. This repressor then inhibits the expression of protein degradation tagged RFP from the downstream No part name specified with partinfo tag. promoter. In the presence of copper, the repression of CueR is inhibited leading to greater RFP expression. Fluorescence of this construct has been shown to increase with increasing copper concentration in both a qualitative and quantitative manner.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1229
Illegal AgeI site found at 1341 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |