Part:BBa_K2333427

UNS pTet mScarlet-I

This part is contained in a suite of protein degradation tagged mScarlet-I reporters under the control of the aTc-inducible pTet promoter combined with the pTet repressor tetR under the medium-weak strength constitutive promoter J23105. These parts were used along with the IPTG-inducible mf-Lon protease to demonstrate distinct levels of speed to steady state in reporter expression proportional to the relative strength of each pdt. This specific part is a tagless inducible control construct (J23105 mScarlet-I with no pdt) which can be used as a comparison against protein degradation for parts with pdt's.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 978
  Illegal NheI site found at 1001
  Illegal NotI site found at 632
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., . . . Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074

[2] Cameron DE, Collins JJ. Tunable protein degradation in bacteria. Nature Biotechnology. 2014;32(12):1276–1281.

[3] Torella JP, Boehm CR, Lienert F, Chen J-H, Way JC, Silver PA. Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic Acids Research. 2013;42(1):681–689.