RNA

Part:BBa_K2254999

Designed by: Ching Yuet To   Group: iGEM17_Hong_Kong-CUHK   (2017-10-19)
Revision as of 08:26, 27 October 2017 by AsaNim (Talk | contribs)


SAT toehold swicth

This is an improved version of previous biobrick BBa_K1728019. The design of original biobrick aims to detect spermine N1-acetyltransferase (SAT) mRNA , which is a biomarker of oral cancer. We improved the biobrick by converting the luciferase reporter gene to mRFP reporter gene, which can be visualized by naked eyes to allow on-site detection. We also demonstrated that it can detect SAT transcript.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 665
    Illegal AgeI site found at 777
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None