RNA

Part:BBa_K2254020

Designed by: Ching Yuet To   Group: iGEM17_Hong_Kong-CUHK   (2017-08-31)
Revision as of 15:19, 31 August 2017 by Registry (Talk | contribs)

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PB2-2 Toehold Switch

PB2-2 Toehold Switch is one of the synthetic toehold switch that is designed to detect the polymerase basic protein 2 (PB2) RNA in influenza A virus. iGEM17 Hong Kong-CUHK team designed 3 toehold switches to detect the Polymerase basic protein 2 (PB2) gene (BBa_K2254010, BBa_K2254020, BBa_K2254030).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 680
    Illegal AgeI site found at 792
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None