Part:BBa_K2333413
UNS J23100 mScarlet-I
This part is contained in a suite of protein degradation tagged mScarlet reporters under the control of the strong constitutive promoter J23100. These parts, in combination with inducible mf-Lon protease constructs, allowed us to characterize the degradation properties of each protein degradation tag (pdt) on a plasmid-based system. We successfully demonstrated distinct levels of protein degradation by each of the 6 pdt’s, and mScarlet reporters have been codon-optomized for E. coli and feature a double stop codon for enhanced efficiency. This specific part is a tagless control construct (J23100 mScarlet with no pdt) which can be used as a comparison against protein degradation for parts with pdt's.
Usage and Biology
This part contains mScarlet-I with no pdt under the control of the constitutive promoter J23100. It contains a double stop codon and BBa_B0015 (double terminator) in the William and Mary iGEM Universal Nucleotide Sequences (UNS) format. This enables easy cloning with Gibson Assembly, as UNS primers are designed for easy PCRs and high yield Gibson Assembly. When used in combination with inducible mf-Lon protease constructs, this part can be used as a control in characterizing degradation properties of the 6 pdt's. This is the first experimentally-demonstrated system that allows future iGEM teams to access modular, predictive control over the temporal dynamics of their circuits by swapping parts at the genetic sequence level.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
Illegal NheI site found at 70
Illegal NotI site found at 605 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |