Part:BBa_K2325001

Phosphite dehydrogenase (PTDH)

The BBa_K2325001 harbors a coding sequence of Phosphite dehydrogenase (PTDH), which catalyzes the conversion of phosphite to phosphate.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

Phosphite dehydrogenase is an enzyme involved in the production of phosphate for providing phosphorus source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of phosphorus in E.coli.

KH₂PO₃ + H₂O + NAD⁺ ⇒ KH₂PO₄ + NADH

Gel analysis

Enzymatic properities

Figure 1: Enzymatic activity of formamidase under different temperatures and pH

Figure 2: The Michaelis constant of formamidase	Figure 3: Influence of metal ion on the enzymatic activity of formamidase