Coding

Part:BBa_K2325001

Designed by: Wenyong Lou   Group: iGEM17_SCUT-FSE-CHINA   (2017-10-14)
Revision as of 14:53, 29 October 2017 by KahiuLau (Talk | contribs)


Phosphite dehydrogenase (PTDH)

The BBa_K2325001 harbors a coding sequence of Phosphite dehydrogenase (PTDH), which catalyzes the conversion of phosphite to phosphate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Phosphite dehydrogenase is an enzyme involved in the production of phosphate for providing phosphorus source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of phosphorus in E.coli.

KH2PO3 + H2O + NAD+ ⇒ KH2PO4 + NADH

Gel analysis

BBa K2325001--1.png

Enzymatic properities

Figure 1: Enzymatic activity of formamidase under different temperatures and pH


Figure 2: The Michaelis constant of formamidase
Figure 3: Influence of metal ion on the enzymatic activity of formamidase

BBa K2325000--4.png

BBa K2325001--2.png
Figure 1.Enzymatic activity of phosphite dehydrogenase under different temperatures and pH


BBa K2325001--3.png
Figure 2.The Michaelis constant of phosphite dehydrogenase


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Categories
Parameters
None