Measurement

Part:BBa_K2406063

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-10-07)
Revision as of 14:18, 7 October 2017 by Registry (Talk | contribs)

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Slox-Term-Vlox Measurement Construct

This part was designed to test if Slox and Vlox Target sites were orthogonal. If either SCre or VCre recombinase could recognise both sites, it would catalyse recombination leading to expression of RFP. If the target sites were orthogonal, no RFP would be produced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 888
    Illegal AgeI site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None