Measurement

Part:BBa_K2406079

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-10-18)
Revision as of 14:17, 18 October 2017 by Registry (Talk | contribs)

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Lox-Term-Rox Measurement Construct

This part was designed to test if Lox and Rox Target sites were orthogonal. If either Cre or Dre recombinase could recognise both sites, it would catalyse recombination leading to expression of RFP. If the target sites were orthogonal, no RFP would be produced

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 886
    Illegal AgeI site found at 998
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None